BD Vacutainer® CPT™ Cell Preparation Tube with Sodium Heparin

Performance Characteristics

Table 2 shows the repeatability of sample preparation using the BD Vacutainer® CPT™ Tube system which was tested and compared to the FICOLL™ HYPAQUE™ method. Ten samples of one donor's pooled blood were centrifuged and assayed in duplicate for each method. No final washing steps were performed. The samples were resuspended to equal final volumes. Estimates of variability due to repeated measurements are shown for within tube and between tubes. Within tube variation was measured by taking duplicate readings from each tube.

Table 2

Repeatability of recovered Mononuclear Cell values, (FICOLL™ HYPAQUE™ versus BD Vacutainer® CPT™ Tube Method) (7)

PARAMETER
	% VARIATION
	AVG	WITHIN TUBE*		BETWEEN TUBE
	%	SD	CV	SD	CV
Recovery
BD Vacutainer® CPT™ (n=9)	79.5	3.41	4.3	6.25	7.9
FICOLL™ HYPAQUE™ (n=10)	73.3	3.55	4.8	2.22	3.0
Purity
BD Vacutainer® CPT™ (n=9)
Total Mononuclear Cells	94.6	0.69	0.7	1.48	1.6
Lymphocytes	72.6	0.93	1.3	0.87	1.2
Monocytes	22.0	0.94	4.3	0.99	4.5
FICOLL™ HYPAQUE™ (n=10)
Total Mononuclear Cells	96.5	0.66	0.7	1.46***	1.5
Lymphocytes	80.5	0.82	1.0	0.91	1.1
Monocytes	16.0	1.06	6.6	1.30	8.2
RBC Contamination
BD Vacutainer® CPT™ (n=9)	28.4	1.36	4.8	13.73	48.5
FICOLL™ HYPAQUE™ (n=10)	14.9	1.21	8.1	5.85	39.3
Granulocyte Contamination
BD Vacutainer® CPT™ (n=9)	5.4	0.62	11.4	1.49	27.3
FICOLL™ HYPAQUE™	3.5	0.57	16.2	1.47	42.1

Footnotes:
*** Within tube variation was determined by taking duplicate readings from each tube.
*** A duplicate reading was lost, so only 9 samples could be compared.
*** With one apparently unusual tube deleted, SD=0.45.
• Parameters defined in Table 1.
• Viability was 100% in all samples since no cell wash steps were performed.
• AVG - Mean Number
• SD - Standard Deviation
• CV - Coefficient of Variation
• n - # of tubes

FICOLL is a trademark of Amersham Biosciences AB
HYPAQUE is a trademark of Amersham Health AS