General BD Vacutainer® Blood Collection Tubes FAQ

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Q.

What type of plastic is used to produce BD Vacutainer® Blood Collection Tubes?

A.

BD Vacutainer® Plus Tubes are made of a special formulation of polyethylene terephthalate (PET). This special formulation is extremely strong, dimensionally stable, and resistant to chemicals and breakage.

Q.

What speed and time should the BD Vacutainer® Blood Collection Tubes be centrifuged at?

A.

All non-gel separator blood collection tubes, including those that contain heparin, EDTA and non-gel serum tubes can be centrifuged at room temperature at a speed of >1300 RCF for 10 minutes.

For coagulation analyses different plasma specifications can be obtained from the citrated blood:
- Platelet-rich plasma: 1500 -2000 g for 5 minutes at 18-25°C
- Platelet-poor plasma: 2,000-2,500 g for 10-15 minutes at 18-25°C
- Platelet-free plasma: > 3,000 g for 15-30 minutes at 18-25°C

The BD Vacutainer® SST and PST gel tubes should be centrifuged at room temperature at a speed of 1000 to 1300 RCF for 10 minutes in a swing bucket centrifuge and 15 minutes in a fixed-angle centrifuge.

The BD Vacutainer® Barricor Plasma Blood Collection Tubes have a mechanical separator and must be centrifuged at a minimum RCF of 1800 for 10 min in a swing bucket centrifuge.

BD Vacutainer® Barricor tubes are optimally centrifuged at an RCF of 4000 for 3 min in a swing bucket centrifuge. BD Vacutainer® Barricor tubes are not compatible with fixed angle centrifuges.

Q.

What coatings layer the walls of BD Vacutainer® Plus Serum and SST tubes?

A.

BD Vacutainer® Plus Plastic Serum and SST tubes are coated with silicone and micronized silica particles to accelerate clotting. A silicone coating reduces adherence of red cells to tube walls. The silica coating can sometimes cause the inner tube wall to appear cloudy and/or filmy. This cloudy appearance is normal and does not affect use.

Q.

How are BD Vacutainer® Blood Collection Tubes packaged?

A.

Most BD Vacutainer® Blood Collection Tubes are packaged 100 tubes/polystyrene tray and 1,000 tubes/case (10 trays).

Q.

Are BD Vacutainer® Blood Collection Tubes endotoxin free?

A.

BD does not claim that any of the BD Vacutainer® Blood Collection Tubes are endotoxin free.

Q.

Do BD Vacutainer® Blood Collection Tubes expire at the beginning or end of the month?

A.

The expiration date on the tube label is stated as a Year/Month/Day.

Q.

How should expired BD Vacutainer® Blood Collection Tubes be disposed of?

A.

Local waste collectors should be consulted to see what the proper method of disposal is for unused, expired blood collection products.

Q.

Are BD Vacutainer® Blood Collection Tubes sterile?

A.

Yes, BD Vacutainer® Blood Collection Tubes have a sterile interior. Tubes are sterilised by gamma irradiation.

Q.

Can BD Vacutainer® Blood Collection Tubes be re-sterilised, for introduction into a sterile field?

A.

BD Vacutainer® Blood Collection Tubes are irradiated to achieve sterility. These tubes are sterile on the interior only. We cannot recommend re-sterilising the tubes, i.e., ETO (ethylene oxide) or autoclaving, primarily due to pressure changes that take place during the re-sterilization cycle. Our tubes are under a specific negative pressure to ensure correct draw volume. During the re-sterilization cycle this negative pressure may be altered and therefore the tubes may not draw correct blood volume.

Q.

Does BD offer any tubes that can be introduced into a sterile field?

A.

BD offers a sterile peel-apart pouch (reference number 366401) that contains a 10 mL glass red top serum tube and 7 mL glass lavender top EDTA tube.

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